Work

Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysis

Year: 2023

Type: article

Abstract: Here, we present a protocol for exploring the effects of PPP1R15A inhibitor, Sephin1, on antitumor immunity of B16F1 subcutaneous tumor in mice. We describe steps for constructing single-cell transcriptome and TCR libraries, sequencing, and using sequencing data for the integration of expression and TCR data. We then detail procedures for gene differentiation, regulon and cell-cell communication analysis, and validation of single-cell analysis results. For complete details on the use and execution of this protocol, please refer to Wang et al.1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2023.102616

Authors: Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu, Ling-Hua Meng, Jingyu Lang, Gang Jin, Yichuan Xiao, Landian Hu, Xiangyin Kong

Institutions: Chinese Academy of Sciences, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Shanghai Jiao Tong University, Shanghai Chest Hospital, Changhai Hospital, ShanghaiTech University, Shanghai Institute of Materia Medica

Language: English

Topic: Cancer Immunotherapy and Biomarkers

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500

Funders: Chinese Academy of Sciences, National Key Research and Development Program of China, National Key Research and Development Program of China Stem Cell and Translational Research

Awards: G2874987640, G3811670283, G4859485, G6049296872, G6623251836, G7705946090

OpenAlex ID: W4387070993

FWCI:

Cites: 6

Cited by:

Related to: 10