Year: 2024
Type: article
Abstract: To address many of the deficiencies in optical neuroimaging technologies, such as poor tempo-spatial resolution, low penetration depth, contact-based measurement, and time-consuming image reconstruction, a novel, noncontact, portable, time-resolved laser speckle contrast imaging (TR-LSCI) technique has been developed for continuous, fast, and high-resolution 2D mapping of cerebral blood flow (CBF) at different depths of the head. TR-LSCI illuminates the head with picosecond-pulsed, coherent, widefield near-infrared light and synchronizes a fast, high-resolution, gated single-photon avalanche diode camera to selectively collect diffuse photons with longer pathlengths through the head, thus improving the accuracy of CBF measurement in the deep brain. The reconstruction of a CBF map was dramatically expedited by incorporating convolution functions with parallel computations. The performance of TR-LSCI was evaluated using head-simulating phantoms with known properties and in-vivo rodents with varied hemodynamic challenges to the brain. TR-LSCI enabled mapping CBF variations at different depths with a sampling rate of up to 1 Hz and spatial resolutions ranging from tens/hundreds of micrometers on rodent head surfaces to 1-2 millimeters in deep brains. With additional improvements and validation in larger populations against established methods, we anticipate offering a noncontact, fast, high-resolution, portable, and affordable brain imager for fundamental neuroscience research in animals and for translational studies in humans.
Authors: Faraneh Fathi, Siavash Mazdeyasna, Dara Singh, Chong Huang, Mehrana Mohtasebi, Xuhui Liu, Samaneh Rabienia Haratbar, Mingjun Zhao, Li Chen, Arin Can Ulku, Paul Mos, Claudio Bruschini, Edoardo Charbon, Lei Chen, Guoqiang Yu
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funders: National Science Foundation, University of Kentucky, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, National Institutes of Health, National Institute of Mental Health, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: Three-Dimensionally Integrated, Ultra-Fast Cameras for Time-Resolved Multi-Wavelength Fluorescence Imaging, Quantum 3D Imaging at high speed and high resolution, Continuous and Longitudinal Monitoring of Cerebral Blood Flow and Metabolism in Freely Moving Rodents, G4628644001, Perioperative Diffuse Optical Imaging of Tissue Blood Flow and Oxygenation for Optimization of Mastectomy Skin Flap Viability, High-density optical tomography of cerebral blood flow and metabolism in small animals, Implementing a novel, multimodal technique for monitoring cerebrovascular hemodynamics in mice as a diagnostic and prognostic tool for single and repeated mild TBI, Noninvasive Noncontact High-Density Optical Imaging of Neonatal Intraventricular Hemorrhage, G7844654242, A Low-cost Compact Diffuse Speckle Contrast Flow-oximeter for Neonatal Brain Monitoring, University of Kentucky Markey Cancer Center – Cancer Center Support Grant, Time-resolved laser speckle contrast imaging of resting-state functional connectivity in neonatal brain, Development of a Wearable Fluorescence Imaging Device for IntraoperativeIdentification of Brain Tumors
OpenAlex ID: W4403722099