Work

Engineering a humanized animal model of polycythemia vera with minimal JAK2V617F mutant allelic burden

Year: 2023

Type: article

Abstract: We used CRISPR/Cas9 to introduce the JAK2 V617F mutation into human stem and progenitor cells to engineer a humanized model of myeloproliferative neoplasm.Data show that polycythemia vera pathologies can manifest from a mutant allele burden of less than 5%, and introduction of the JAK2 V617F mutation into older age stem cells has predilection for fibrotic progression. Disclosures of conflicts of

Source: Haematologica

DOI: 10.3324/haematol.2023.283858

Authors: Tyler M. Parsons, Aishwarya Krishnan, Wangisa M.B. Dunuwille, Andrew L. Young, Jason Arand, Wentao Han, Grant A. Challen

Institution: Washington University in St. Louis

Language: English

Topic: Myeloproliferative Neoplasms: Diagnosis and Treatment

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,157

Funders: Leukemia and Lymphoma Society, MPN Research Foundation, Incyte, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: Functions of JARID2 in Normal and Neoplastic Hematopoiesis, JAK/STAT signaling in the pathogenesis of DNMT3A mutant T-ALL, Pre-and Postgraduate Training in Molecular Hematology, Inflammatory Stress Promotes Clonal Expansion of DNMT3A-mutant HSCs, Cancer Center Support Grant

OpenAlex ID: W4387115851

FWCI:

Cites: 15

Cited by:

Related to: 10