Decreased Transition-State Analogue Affinity in Isotopically Heavy MTAN with Increased Catalysis
Year: 2023
Type: article
Abstract: 5′-Methylthioadenosine/ S -adenosylhomocysteine nucleosidase from Helicobacter pylori ( Hp MTAN) demonstrated faster chemistry when expressed as an isotopically heavy protein, with 2 H, 13 C, and 15 N replacing the bulk of normal isotopes. The inverse heavy enzyme isotope effect has been attributed to improved enzyme–reactant interactions causing more frequent transition-state formation ( Proc. Natl. Acad. Sci. U.S.A. 2021, 118, e2109118118 ). Transition-state analogues stabilize the transient dynamic geometry of the transition state and inform on transition-state dynamics. Here, a slow-onset, tight-binding transition-state analogue of Hp MTAN is characterized with heavy and light enzymes. Dissociation constants for the initial encounter complex ( K i ) and for the tightly bound complex after slow-onset inhibition ( K i *) with hexylthio-DADMe-Immucillin-A (HTDIA) gave K i values for light and heavy Hp MTAN = 52 ± 10 and 85 ± 13 pM and K i * values = 5.9 ± 0.3 and 10.0 ± 1.2 pM, respectively. HTDIA dissociates from heavy Hp MTAN at 0.063 ± 0.002 min –1, faster than that from light Hp MTAN at 0.032 ± 0.004 min –1 . These values are consistent with transition-state formation by an improved catalytic site dynamic search and inconsistent with catalytic efficiency proportional to tight binding of the transition state.
Source: Biochemistry
Authors: Morais Brown, Vern L. Schramm
Institution: Albert Einstein College of Medicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4387312113