A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells
Year: 2023
Type: article
Abstract: Cellular senescence is a stress-response mechanism implicated in various physiological processes, diseases, and aging. Current detection approaches have partially addressed the issue of senescent cell identification in clinical specimens. Effective methodologies enabling precise isolation or live tracking of senescent cells are still lacking. In-depth analysis of truly senescent cells is, therefore, an extremely challenging task. We report (1) the synthesis and validation of a fluorophore-conjugated, Sudan Black-B analog (GLF16), suitable for in vivo and in vitro analysis of senescence by fluorescence microscopy and flow cytometry and (2) the development and application of a GLF16-carrying micelle vector facilitating GLF16 uptake by living senescent cells in vivo and in vitro. The compound and the applied methodology render isolation of senescent cells an easy, rapid, and precise process. Straightforward nanocarrier-mediated GLF16 delivery in live senescent cells comprises a unique tool for characterization of senescence at an unprecedented depth.
Source: Molecular Cell
Authors: Sophia Magkouta, Dimitris Veroutis, Athanasios Pousias, Angelos Papaspyropoulos, Natassa Pippa, Nikolaos Lougiakis, Konstantinos Kambas, Nefeli Lagopati, Aikaterini Polyzou, Maria Georgiou, Maria Chountoulesi, Stergios Pispas, Spyros Foutadakis, Nicole Pouli, Panagiotis Marakos, Athanassios Kotsinas, Panayotis Verginis, Dimitrios Valakos, Athanasia Mizi, Argyris Papantonis, Giannis Vatsellas, Panagiotis Galanos, Jiri Bartek, Russell Petty, Manuel Serrano, Dimitris Thanos, Charis Roussos, Marco Demaria, Konstantinos Evangelou, Vassilis G. Gorgoulis
Institutions: National and Kapodistrian University of Athens, Evangelismos Hospital, Academy of Athens, Biomedical Research Foundation of the Academy of Athens, National Hellenic Research Foundation, Pasteur Hellenic Institute, University of Crete, FORTH Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Universitätsmedizin Göttingen, Danish Cancer Society, Science for Life Laboratory, Karolinska Institutet, University of Dundee, Ninewells Hospital, Institute for Research in Biomedicine, Barcelona Institute of Science and Technology, University Medical Center Groningen, Manchester Academic Health Science Centre, University of Surrey, University of Manchester
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $9,080
OpenAlex ID: W4387364608