Year: 2023
Type: article
Abstract: The ability to monitor intracellular calcium concentrations using fluorescent probes has led to important insights into biological signaling processes at the cellular level. An important challenge is to relate such measurements to broader patterns of signaling across fields of view that are inaccessible to optical techniques. To meet this need, we synthesized molecular probes that couple calcium-binding moieties to lanthanide texaphyrins, resulting in complexes endowed with a diverse complement of magnetic and photophysical properties. We show that the probes permit intracellular calcium levels to be assessed by fluorescence, photoacoustic, and magnetic resonance imaging modalities and that they are detectable by multimodal imaging in brain tissue. This work thus establishes a route for monitoring signaling processes over a range of spatial and temporal scales.
Source: ACS Sensors
Authors: Grégory D. Thiabaud, Miriam Schwalm, Sajal Sen, Ali Barandov, Jacob Simon, Peter Harvey, Virginia Spanoudaki, Peter Müller, Jonathan L. Sessler, Alan Jasanoff
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funders: Welch Foundation, Massachusetts Institute of Technology, Wellcome Trust, National Institutes of Health, Simons Center for the Social Brain, Massachusetts Institute of Technology, National Eye Institute, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering
OpenAlex ID: W4387448682