Work

Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis

Year: 2023

Type: preprint

Abstract: Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

Source: Cold Spring Harbor Protocols

DOI: 10.1101/pdb.prot108194

Authors: Vanessa Bottino-Rojas, Anthony A. James

Institution: University of California, Irvine

Language: English

Topic: Mosquito-borne diseases and control

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: University of California, Irvine, National Institute of Allergy and Infectious Diseases

Awards: Expression of Exogenous Genes in Vector Mosquitoes, Impact of gene-drive systems for population modification on malaria vector mosquitoes