Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
Year: 2023
Type: article
Abstract: Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity. For complete details on the use and execution of this protocol, please refer to Hageter et al. (2023).1
Source: STAR Protocols
Institution: West Virginia University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Awards: G6744335439, G7128002086, Examining how visual experience modulates the thalamus and visuomotor responses., RII Track 1: West Virginia Network for Functional Neuroscience and Transcriptomics (WV-NFNT), Cell and Molecular Biology Training Program at West Virginia University, Visual Sciences Center of Biomedical Research Excellence, How transposable elements drive genome evolution through epigenetic mechanisms
OpenAlex ID: W4387631272