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Call to action to properly utilize electron microscopy to measure organelles to monitor disease

Year: 2023
Type: review
Abstract: This review provides an overview of the current methods for quantifying mitochondrial ultrastructure, including cristae morphology, mitochondrial contact sites, and recycling machinery and a guide to utilizing electron microscopy to effectively measure these organelles. Quantitative analysis of mitochondrial ultrastructure is essential for understanding mitochondrial biology and developing therapeutic strategies for mitochondrial-related diseases. Techniques such as transmission electron microscopy (TEM) and serial block face-scanning electron microscopy, as well as how they can be combined with other techniques including confocal microscopy, super-resolution microscopy, and correlative light and electron microscopy are discussed. Beyond their limitations and challenges, we also offer specific magnifications that may be best suited for TEM analysis of mitochondrial, endoplasmic reticulum, and recycling machinery. Finally, perspectives on future quantification methods are offered.
DOI: 10.1016/j.ejcb.2023.151365
Authors: Kit Neikirk, Edgar-Garza Lopez, Andrea G. Marshall, Ahmad Alghanem, Evan Krystofiak, Bartosz Kula, Nathan Smith, Jianqiang Shao, Prasanna Katti, Antentor Hinton
Institutions: Vanderbilt University, University of Iowa, King Abdullah International Medical Research Center, University of Rochester Medicine, University of Rochester, National Institutes of Health, National Heart, Lung, and Blood Institute
Language: English
Open Access status: gold
Estimated APC paid: $2,420
Funders: National Science Foundation, Burroughs Wellcome Fund, Silicon Valley Community Foundation, Chan Zuckerberg Initiative, National Institutes of Health, Vanderbilt Diabetes Research and Training Center, Vanderbilt University Medical Center, National Institute of Neurological Disorders and Stroke, United Negro College Fund
Awards: G2393974219, Astrocytes Modulate Synaptic Plasticity via Norepinephrine, G5656976469, PRIDE-Functional and Translational Genomics of Blood Disorders, NCS-FR: Protecting the Aging Brain: Self-Organizing Networks and Multi-Scale Dynamics under Energy Constraints, G9000052735
OpenAlex ID: W4387675569
FWCI: 1.539
Cites: 146
Cited by: 58
Related to: 10