Calligraphy of Nanoplasmonic Bioink-Based Multiplex Immunosensor for Precision Immune Monitoring and Modulation
Year: 2023
Type: article
Abstract: Immunomodulation therapies have attracted immense interest recently for the treatment of immune-related diseases, such as cancer and viral infections. This new wave of enthusiasm for immunomodulators, predominantly revolving around cytokines, has spurred emerging needs and opportunities for novel immune monitoring and diagnostic tools. Considering the highly dynamic immune status and limited window for therapeutic intervention, precise real-time detection of cytokines is critical to effectively monitor and manage the immune system and optimize the therapeutic outcome. The clinical success of such a rapid, sensitive, multiplex immunoanalytical platform further requires the system to have ease of integration and fabrication for sample sparing and large-scale production toward massive parallel analysis. In this article, we developed a nanoplasmonic bioink-based, label-free, multiplex immunosensor that can be readily "written" onto a glass substrate via one-step calligraphy patterning. This facile nanolithography technique allows programmable patterning of a minimum of 3 μL of nanoplasmonic bioink in 1 min and thus enables fabrication of a nanoplasmonic microarray immunosensor with 2 h simple incubation. The developed immunosensor was successfully applied for real-time, parallel detection of multiple cytokines (e.g., interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-beta (TGF-β)) in immunomodulated macrophage samples. This integrated platform synergistically incorporates the concepts of nanosynthesis, nanofabrication, and nanobiosensing, showing great potential in the scalable production of label-free multiplex immunosensing devices with superior analytical performance for clinical applications in immunodiagnostics and immunotherapy.
Authors: Jiacheng He, Siqi Wu, Wu Chen, Albert Kim, Wen Yang, Chuanyu Wang, Zhengyang Gu, Jialiang Shen, Siyuan Dai, Weiqiang Chen, Pengyu Chen
Institutions: Auburn University, New York University, Vanderbilt University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: National Science Foundation, Alabama EPSCoR, National Institutes of Health, American Chemical Society Petroleum Research Fund, National Institute of General Medical Sciences, Office of Integrative Activities, Division of Chemical, Bioengineering, Environmental, and Transport Systems, Office of Experimental Program to Stimulate Competitive Research
Awards: CAREER: Nano-Plasmon Ruler Imaging for Direct Visualization of How Cells "Talk", G2149557165, G3154264318, Bioengineering Leukemic Bone Marrow Niche for Dissecting the Heterogeneous Leukemia Chemo-resistance Mechanisms, G6459979171, G6671297155, RII Track-4: van der Waals polaritonics for mid-infrared light emitters, G725005689, G7484750973, Dissecting and engineering CAR T-cell function for optimized Immunotherapy, G8439143919, Machine Learning-Assisted Integrated Optofluidic Nanoplasmonic Biosensing for Precision Immune Profiling and Monitoring
OpenAlex ID: W4387771493