Work

Prospective isolation of neural stem and progenitor cells from the developing human brain

Year: 2023

Type: article

Abstract: Prospective isolation of defined cell types is critical for the functional study of stem cells, especially in primary human tissues. Here, we present a protocol for purifying 10 transcriptomically and functionally distinct neural stem and progenitor cell types from the developing human brain using fluorescence-activated cell sorting. We describe steps for tissue dissociation, staining, and cell sorting as well as downstream functional experiments for measuring clonogenicity, differentiation, and engraftment potential of purified populations. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2023.102674

Authors: Daniel Dan Liu, Joy Q. He, Nobuko Uchida, Irving L. Weissman, Rahul Sinha

Institutions: Stanford Medicine, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University

Language: English

Topic: Neurogenesis and neuroplasticity mechanisms

Subfield: Developmental Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500