CG -SLENP: A Chemical Genetics Strategy To Selectively Label Existing Proteins and Newly Synthesized Proteins
Year: 2024
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Protein synthesis and subsequent delivery to the target locations in cells are essential for their proper functions. Methods to label and distinguish newly synthesized proteins from existing ones are critical to assess their differential properties, but such methods are lacking. We describe the first c hemical g enetics-based approach for s elective l abeling of e xisting and n ewly synthesized p roteins that we termed as CG -SLENP. Using HaloTag in-frame fusion with lamin A (LA), we demonstrate that the two pools of proteins can be selectively labeled using CG -SLENP in living cells. We further employ our recently developed selective small molecule ligand LBL1 for LA to probe the potential differences between newly synthesized and existing LA. Our results show that LBL1 can differentially modulate these two pools of LA. These results indicate that the assembly states of newly synthesized LA are distinct from existing LA in living cells. The CG -SLENP method is potentially generalizable to study any cellular proteins.
Source: JACS Au
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,500
Awards: G1067399340, Chemical tools to decode nuclear lamina-ome, Synergistic Inhibitors of CREB-mediated Gene Transcription, Chemical Modulators of Nuclear Lamins, Targeting EWS-ATF1 Fusion in Clear Cell Sarcoma of Soft Tissue, OHSU Knight Cancer Institute, Small molecules targeting RuvBL complex for triple negative breast cancer
OpenAlex ID: W4400975507