Work

Reconstitution of membrane contact by unilamellar vesicles

Year: 2023

Type: article

Abstract: Eukaryotic cells compartmentalize diverse biochemical functions within organelles defined by intracellular membranes. Recent focus has intensified on studying the interactions among organelles and the role of membrane contacts in maintaining cellular balance. While analyzing these contacts mainly involves fluorescence and electron microscopy, as well as biochemical cell fractionation, understanding their mechanisms and responses to genetic and environmental changes remains challenging. Here we describe an approach employing in vitro reconstitution of membrane contacts using unilamellar vesicles. This technique offers insights into contact mechanisms when combined with established methods like fluorescence imaging and mass spectrometry, potentially deepening our understanding of membrane contacts and organelle networks.

Source: Biophysics Reports

DOI: 10.52601/bpr.2023.230011

Authors: Shulin Li, Min Zhang, Liang Ge

Institutions: Center for Life Sciences, State Key Laboratory of Membrane Biology, Tsinghua University

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: National Natural Science Foundation of China

Awards: G2079023696, G6515829220, G7718699922, G775703907