FIGURE 3 from PRMT5 Inhibitors Regulate DNA Damage Repair Pathways in Cancer Cells and Improve Response to PARP Inhibition and Chemotherapies
Year: 2023
Type: other
Abstract: C220 dysregulates chromatin- and splicing-mediated regulation of DDR pathways. A, ChIP-qPCR assays using primers against the proximal promoter of indicated DDR genes following capture of chromatin-bound PRMT5 in nontreated MCF7 cells. Data are shown as fold enrichment over IgG (n = 3). B, ChIP-qPCR assays using primers against the proximal promoter of DDR genes following capture of chromatin-bound H4R3me2 from MCF7 cells treated with 250 nmol/L C220 for 4 days. Data are shown as % input (n = 3) C, AS analysis of RNA-seq data from MCF7, HCC1569, and A2780 cells following treatment with 100 nmol/L C220 for 4 days. Total events represent the total number of AS with statistical significance (P < 0.05). RI = retained intron, SE = skipped exon, A3 = alternative 3′ splice-site, A5 = alternative 5′ splice site, MX = mutually exclusive exons, AF = alternative first exon, AL = alternative last exon. D, Violin plot of global intron retention and exon skipping events induced by C220 in A2780 cells annotated with select DDR genes with significant (P < 0.05) events. E and F, qPCR of total ATM or ATM retained intron in A2780 cells treated with 100 nmol/L C220 for 4 days.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4388424162