FIGURE 2 from PRMT5 Inhibitors Regulate DNA Damage Repair Pathways in Cancer Cells and Improve Response to PARP Inhibition and Chemotherapies
Year: 2023
Type: other
Abstract: C220 broadly suppresses DDR Gene expression in ovarian and breast cancer cells. A, Volcano plots from RNA-seq data in breast (MCF7) and ovarian (A2780) cancer cell lines treated with 100 nmol/L C220 for 4 days (n = 3). Select DDR genes are highlighted. B, Top eight downregulated hits from KEGG pathway analysis of significantly DEGs from MCF7 and A2780 RNA-seq data (q value < 0.01, n = 3). C, Clustered heat map of HRD signature genes in C220-treated MCF7 cells (n = 3). D, Dose-dependent downregulation of select DDR genes was validated by qRT-PCR (fold change ddCT from DMSO control) following treatment with C220 for 4 days (n = 3). Data are represented as mean ± SEM. E and F, Western blots highlighting dose-dependent downregulation of select DDR proteins following treatment with C220 for 4 days (n = 2).
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4388424390