Year: 2023
Type: editorial
Abstract: Editorial on the Research Topic Plant-microbe omicsOmics-based studies have evolved over the past two decades to provide rich datasets from which deeper system-level understanding can be unraveled.Omics technologies (e.g., genomics, transcriptomics, proteomics, and metabolomics) can provide specific molecular insights into plant and microbe fitness, disease states, stress, cell signaling/cell-cell communication, and nutrient exchange, and these molecular observations can be correlated with system phenotype and function.The overall health of plant-microbe systems and their surrounding environments can be assessed, and a greater understanding of processes related to nutrient exchange/cycling, plant disease, and ecosystem homeostasis can now be achieved via the latest approaches to plant-microbe omics analysis.Plant-microbe interactions have traditionally been some of the most technically challenging sample types for omics analysis.Plant tissues, which contain recalcitrant cell wall polymers, make the extraction of biomolecules (i.e., proteins, transcripts, and metabolites) more involved compared to samples such as those analyzed in human/ animal systems or clinical samples.Plant samples notoriously contain high levels of polyphenolics or other reactive compounds that, if not treated and removed properly from the protein samples, can negatively react with and alter metabolite and protein structure.Samples extracted from the rhizosphere often contain high levels of humic and fulvic acids, which are difficult to remove from the sample biomolecules of interest and which can negatively interfere with successful mass spectrometric or sequencing analyses, such as those used in proteomics and transcriptomics, for example.The aim of this Research Topic, therefore, was to cover current research in multi-omics applied specifically to elucidating plant-microbe interactions.This Research Topic, consisting of four articles, includes omics studies on plants interacting symbiotically or antagonistically (i.e., through pathogenic infection) with microorganisms.The research encompasses metatranscriptomics analyses of rhizosphere soils, bottom-up proteomic approaches, along with more specialized proteomic analyses such as top-down proteomics analyses that investigate post-translational modifications, and single-cell omics analyses to probe biomolecular changes at the cellular level to elucidate various plant-microbe interactions.
Source: Frontiers in Analytical Science
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,125
OpenAlex ID: W4388456237