FIGURE 2 from Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans
Year: 2023
Type: other
Abstract: Relevance of antibodies detected by immunoassay to L1 ORF1p. The results of immunofluorescent staining (A) and immunoblot analysis (B) of HeLa cells transduced with Tet-inducible L1/Gluc reporter construct with (+DOX) and without (−DOX) L1 induction by doxycycline (Supplementary Fig. S1). “Rabbit αORF1p Ab” – polyclonal anti-ORF1p rabbit antiserum. The other panels represent staining with 50,000-fold diluted serum samples from patients with ovarian cancer with 107–108 anti-ORF1p IgG titers in anti-ORF1p immunoassay. “M” stands for the protein size marker added to the sample along with HeLa cells lysate without DOX L1 induction. The asterisks on the bottom panel correspond to ORF1p standard (40-kDa) band.
Authors: Alexandra V. Vylegzhanina, Ivan A. Bespalov, Ksenia A. Novototskaya-Vlasova, Brandon M. Hall, Anatoli S. Gleiberman, Han Yu, Olga V. Leontieva, Katerina I. Leonova, Oleg V. Kurnasov, Andrei L. Osterman, Grace K. Dy, Alexey A. Komissarov, Elena Vasilieva, Jeff Gehlhausen, Akiko Iwasaki, Christine B. Ambrosone, Takemasa Tsuji, Junko Matsuzaki, Kunle Odunsi, Ekaterina L. Andrianova, Andrei V. Gudkov
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4388490275