Distinguishing Protein Chemical Topologies Using Supercharging Ion Mobility Spectrometry‐Mass Spectrometry
Year: 2023
Type: article
Abstract: A technique combining ion mobility spectrometry‐mass spectrometry (IMS‐MS) and supercharging electrospray ionization (ESI) has been demonstrated to differentiate protein chemical topology effectively. Incorporating as many charges as possible into proteins via supercharging ESI allows the protein chains to be largely unfolded and stretched, revealing their hidden chemical topology. Different chemical topologies result in differing geometrical sizes of the unfolded proteins due to constraints in torsional rotations in cyclic domains. By introducing new topological indices, such as the chain‐length‐normalized collision cross‐section (CCS) and the maximum charge state (zM) in the extensively unfolded state, we were able to successfully differentiate various protein chemical topologies, including linear chains, ring‐containing topologies (lasso, tadpole, multicyclics, etc.), and mechanically interlocked rings, like catenanes.
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
Funders: Kenyon Review, Innovative Research Group Project of the National Natural Science Foundation of China, National Research Foundation, National Natural Science Foundation of China, National Research Foundation of Korea, Ministry of Science and ICT, South Korea, Key Technologies Research and Development Program, Beijing National Laboratory for Molecular Sciences
Awards: G1610288917, G1697327937, G2160034316, G2517259798, G2578945855, G2616059945, G319524719, G3784052911, G3794665687, G4151770711, G4184608533, G430625229, G4808871591, G5275026023, G5470924738, G5820275321, G5919250277, G6132202710, G6287779381, G7549294502, G7769691814, G7906011681, G7916842217, G8366542191, G8614702290
OpenAlex ID: W4388499818