FIGURE 2 from Enitociclib, a Selective CDK9 Inhibitor, Induces Complete Regression of MYC+ Lymphoma by Downregulation of RNA Polymerase II Mediated Transcription
Year: 2023
Type: other
Abstract: Enitociclib depletes mRNA transcripts, MYC and MCL1; downregulates Myc and Mcl-1 protein levels and activates cell death in vitro MYC (A) and MCL1 (B) mRNA levels were determined using qPCR normalized to 18s rRNA from SU-DHL-10 and SU-DHL-4 DLBCL cells treated with indicated concentration of CDK9 inhibitor for 4 hours. The protein levels of Myc (C) and Mcl-1 (D) pretreatment and at indicated times after treatment washout were quantified relative to GAPDH in SU-DHL-10 and SU-DHL-4 cells. The inset shows Myc protein levels in SU-DHL-10 cells until 8 hours after treatment. E, The protein levels of cPARP is quantified from SU-DHL-10 DLBCL cells pretreatment and at indicated times after treatment.
Language: English
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4388525835