PB0163 Differential Binding of Recombinant FVIII Products to Platelets, and Impact of FVIII on VWF Expression and Platelet Contractility In Vitro
Year: 2023
Type: conference-abstract
Abstract: Background: Binding of factor VIII (FVIII) to procoagulant platelets ensures efficient localisation and availability of thrombin at injury sites.It is unclear whether modifications in recombinant FVIII (rFVIII) products impact FVIIIplatelet binding and subsequent platelet signalling.Aims: To examine binding of different rFVIII products to platelets in vitro, and the impact of FVIII on von Willebrand factor (VWF) expression and platelet contractility.Methods: Platelet-FVIII binding: Platelets isolated from healthy donors and people with haemophilia A were activated with thrombin and cross-linked collagen-related peptide (CRP-XL), and incubated with simoctocog alfa (Nuwiq ® ), efmoroctocog alfa, rurioctocog alfa pegol or damoctocog alfa pegol.Binding was quantified by FVIII immunostaining and flow cytometry, and values were normalised to values for simoctocog alfa.Furthermore, identification of FVIII binding receptors using TriCEPS-based ligandreceptor capture technology was conducted.VWF expression: Isolated platelets were seeded before activation with adenosine diphosphate (ADP),
Institution: ETH Zurich
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,250
OpenAlex ID: W4388598091