Work

Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation

Year: 2023

Type: article

Abstract: S-adenosylmethionine-dependent methyltransferases are involved in countless biological processes, including signal transduction, epigenetics, natural product biosynthesis, and detoxification. Only a handful of carboxylate methyltransferases have evolved to participate in amide bond formation. In this report we show that enzyme-catalyzed F-methylation of carboxylate substrates produces F-methyl esters that readily react with N- or S-nucleophiles under physiological conditions. We demonstrate the applicability of this approach to the synthesis of small amides, hydroxamates, and thioesters, as well as to site-specific protein modification and native chemical ligation.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.202312104

Authors: Jiaming Peng, Gregory R. Hughes, Manuel M. Müller, Florian P. Seebeck

Institutions: University of Basel, King's College London

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $5,250