Work

3-D culture of human lung fibroblasts decreases proliferative and increases extracellular matrix remodeling genes

Year: 2023

Type: article

Abstract: With the introduction of complex three-dimensional (3-D) lung models, comes a need for understanding cellular behavior in these models. We compared gene expression profiles of human lung fibroblasts grown on two-dimensional (2-D) collagen I-coated surfaces with those in 3-D collagen I hydrogels. RNA sequencing and subsequent pathway analyses showed decreased proliferation, increased extracellular matrix (ECM) remodeling, and altered Hippo signaling and ECM deposition-related gene signatures. These findings highlight unique responses of fibroblasts in 3-D models.

Source: American Journal of Physiology-Cell Physiology

DOI: 10.1152/ajpcell.00374.2023