Work

Integrative modeling of JCVI-Syn3A nucleoids with a modular approach

Year: 2023

Type: article

Abstract: A lattice-based method is presented for creating 3D models of entire bacterial nucleoids integrating ultrastructural information cryoelectron tomography, genomic and proteomic data, and experimental atomic structures of biomolecules and assemblies. The method is used to generate models of the minimal genome bacterium JCVI-Syn3A, producing a series of models that test hypotheses about transcription, condensation, and overall distribution of the genome. Lattice models are also used to generate atomic models of an entire JCVI-Syn3A cell.

Source: Current Research in Structural Biology

DOI: 10.1016/j.crstbi.2023.100121

Authors: David S. Goodsell, Ludovic Autin

Institutions: Rutgers, The State University of New Jersey, Scripps Research Institute

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,500

Funders: National Institutes of Health, National Institute of General Medical Sciences