Knockdown of miR-27a reduces TGFβ-induced EMT and H2O2-induced oxidative stress through regulating mitochondrial autophagy.
Year: 2023
Type: article
Abstract: OBJECTIVES: The present research aimed at clarifying the role played by miR-27a in the context of intrauterine adhesion (IUA) by focusing on its impact on TGFβ1-induced epithelial-mesenchymal transition (EMT), migration, oxidative stress, and mitochondrial autophagy in endometrial stromal cells (ESCs). METHODS: We employed the Cell Counting Kit CCK-8/WST-8 assay to assess ESC proliferation, flow cytometric analysis and an Annexin-V-FITCV-FITC Apoptosis Detection kit to determine cell apoptosis, and wound healing and transwell assays to evaluate cell migration. Besides, intracellular reactive oxygen species (ROS) levels measured by the Reactive Oxygen Species Assay Kit were analyzed by flow cytometry, and protein expression levels were quantified by Western blotting analysis. RESULTS: -induced EMT through PINK1. CONCLUSIONS: induction. Our findings highlight miR-27a as a potential therapeutic target for IUA treatment, shedding light on its multifaceted involvement in the mechanism of intrauterine adhesion fibrosis.
Source: PubMed Central
Authors: Fangfang Zhao, Wei Wei, Dongping Huang, Yi Guo
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4388737051