FIGURE 3 from Concurrent Targeting of HDAC and PI3K to Overcome Phenotypic Heterogeneity of Castration-resistant and Neuroendocrine Prostate Cancers
Year: 2023
Type: other
Abstract: Transcriptional changes associated with fimepinostat treatment are largely conserved between CRPC and NEPC. A, Venn diagrams showing overlapping upregulated and downregulated genes in the 22Rv1 and LNCaP95 cell lines after treatment with 1 µmol/L fimepinostat relative to treatment with 0.1% DMSO for 24 hours. B, Plots showing commonly enriched (top, yellow bars) and de-enriched (bottom, blue bars) MSigDB hallmark gene sets from GSEA of overlapping genes in A. C, Gene expression heat map of 22Rv1 and LNCaP95 cells treated with DMSO or increasing doses of fimepinostat for 24 hours. Select genes including Myc, AR, AR targets, NE markers, and EMT markers are shown. D, Venn diagrams showing overlapping upregulated and downregulated genes in the NCI-H660 and MSKCC EF1 cell lines after treatment with 1 µmol/L fimepinostat relative to treatment with 0.1% DMSO for 24 hours. E, Plots showing commonly enriched (top, yellow bars) and de-enriched (bottom, blue bars) MSigDB hallmark gene sets from GSEA of overlapping genes in D. F, Gene expression heat map of NCI-H660 or MSKCC EF1 cells treated with DMSO or increasing doses of fimepinostat for 24 hours. Select genes including Myc paralogs and NE transcription factors are shown. G, Plot showing the overall changes in gene expression of transcription factors after treatment with 1 µmol/L fimepinostat relative to treatment with 0.1% DMSO for 24 hours. Select highly enriched and de-enriched transcription factors are labeled.
Authors: Ailin Zhang, Nathan A. Lau, Alicia Wong, Lisha G. Brown, Ilsa M. Coleman, Navonil De Sarkar, Dapei Li, Diana C. DeLucia, Mark P. Labrecque, Holly M. Nguyen, Jennifer L. Conner, Ruth F. Dumpit, Lawrence D. True, Daniel W. Lin, Eva Corey, Joshi J. Alumkal, Peter S. Nelson, Colm Morrissey, John K. Lee
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4388825829