Year: 2023
Type: preprint
Abstract: This protocol accompanies the manuscript Mapping Human Tissues with Highly Multiplexed RNA in situ Hybridization (https://doi.org/10.1101/2023.08.16.553610). It protocol outlines the steps for the enzymatic amplification and preparation of padlock probes from an oligo pool. This strategy has a lower upfront cost compared to individual synthesis of probes and thus permits the use of tens of thousands of padlock probes per probe set. Additionally, the oligo pool serves as an unlimited source of padlock probes, as it can be expanded by PCR amplification upon need.
Authors: Chien-Ju Chen, Kian Kalhor, Kun Zhang
Institution: University of California San Diego
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4388916750