Year: 2023
Type: article
Abstract: Neuroinflammation causes neuronal injury in multiple sclerosis (MS) and other neurological diseases. MicroRNAs (miRNAs) are important modulators of neuronal stress responses, but knowledge about their contribution to neuronal protection or damage during inflammation is limited. Here, we constructed a regulatory miRNA–mRNA network of inflamed motor neurons by leveraging cell type–specific miRNA and mRNA sequencing of mice undergoing experimental autoimmune encephalomyelitis (EAE). We found robust induction of miR-92a in inflamed spinal cord neurons and identified cytoplasmic polyadenylation element-binding protein 3 ( Cpeb3 ) as a key target of miR-92a–mediated posttranscriptional silencing. We detected CPEB3 repression in inflamed neurons in murine EAE and human MS. Moreover, both miR-92a delivery and Cpeb3 deletion protected neuronal cultures against excitotoxicity. Supporting a detrimental effect of Cpeb3 in vivo, neuron-specific deletion in conditional Cpeb3 knockout animals led to reduced inflammation-induced clinical disability in EAE. Together, we identified a neuroprotective miR-92a– Cpeb3 axis in neuroinflammation that might serve as potential treatment target to limit inflammation-induced neuronal damage.
Source: Science Advances
Authors: Iris Winkler, Jan Broder Engler, Vanessa Vieira, Simone Bauer, Yi-Hsiang Liu, Giovanni Di Liberto, Katarzyna M. Grochowska, Ingrid Wagner, Jasmina Bier, Lukas C. Bal, Nicola Rothammer, Nina Meurs, Kristof Egervari, Benjamin Schattling, Gabriela Salinas, Michael R. Kreutz, Yi-Shuian Huang, Ole Pless, Doron Merkler, Manuel A. Friese
Institutions: University Medical Center Hamburg-Eppendorf, Institute of Neuroimmunology and Multiple Sclerosis, Institute of Biomedical Sciences, Academia Sinica, Academia Sinica, University of Geneva, University Hospital of Geneva, Leibniz Institute for Neurobiology, Universitätsmedizin Göttingen, Fraunhofer Institute for Translational Medicine and Pharmacology
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $4,649
OpenAlex ID: W4388966688