FIGURE 4 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Year: 2023
Type: other
Abstract: Genetically overexpressing Sox2 and chemically replacing Sox2 inhibit Kras-induced dual-positive cell development and tumor formation in alveolar type II cells. A, Sections of Sftpc-CreER; LSL-KrasG12D and Sftpc-CreER; LSL-KrasG12D; LSL-Sox2 mouse lungs at 2, 7, and 12 weeks after tamoxifen injection stained with H&E staining. Tumor area is marked with black arrows. Sftpc-CreER; LSL-KrasG12D—right panel shows higher magnification of boxed area in left panels. B, Quantification of tumors observed after 2 weeks after tamoxifen treatment in Sftpc-CreER; LSL-KrasG12D and Sftpc-CreER; LSL-KrasG12D; LSL-Sox2 mice. Bar graph represents data from 5 mice from each genotype. C, Representative IF images showing Sox2 (green) and Sftpc (red) staining of mouse lung sections in Sftpc-CreER; LSL-KrasG12D and Sftpc-CreER; LSL-KrasG12D; LSL-Sox2 mice. Note the Sox2+ hyperplastic areas (marked with white asterisks) do not express double-positive markers (Sftpc+/Rage+; right). D, Representative H&E images showing the effect of RepSox treatment on the lung of Sftpc-CreER; LSL-KrasG12D; fGFP mice. RepSox or PBS was administered intraperitoneally for 3 weeks (two times/week) 1 week after tamoxifen treatment. Mouse lungs were harvested after 2 weeks of RepSox treatment. E, Quantitative analysis of D using ImageJ software (n = 8 for each group). F, Kaplan–Meier curve showing survival for RepSox versus PBS treatment in Sftpc-CreER; LSL-KrasG12D; fGFP mice [(PBS, n = 11) and (RepSox, n = 14)]. A and B represents data from 5 different mice. Results shown in C are representative of three independent experiments. Error bars represent the mean ± SEM values and significance is defined as *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001.
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4388970326