Work

Protective effect of steroidal saponins on heat stress in the liver of largemouth bass (Micropterus salmoides) revealed by metabolomic analysis

Year: 2023

Type: article

Abstract: With global warming summer high water temperature events are becoming more frequent, and the fragile liver of the largemouth bass (Micropterus salmoides) is particularly vulnerable to heat stress. In this experiment, a metabolomic analysis was conducted to reveal how steroidal saponins alleviated high temperature stress in largemouth bass. Fish were divided into three groups C, H and HS. Fish in groups C and H were fed the basal diet without steroid saponins and group HS were fed the basal diet supplied with 0.1% steroid saponins. After 4-week of breeding for all three groups, the H and HS groups were subjected to 1-week of heat stress, respectively. Metabolomic analysis showed that high temperature stress mainly affected phospholipid metabolism, fatty acid metabolism, glutathione metabolism and bile metabolism in the livers of largemouth bass. In addition, antioxidant enzyme activity and inflammation genes in the livers of largemouth bass showed that high temperature stress reduced the expression of glutathione peroxidase and heme oxygenase-1 and activated inflammation by activating nuclear factor kappa-B. In the HS group, these adverse conditions were greatly improved and phospholipid metabolism, fatty acid metabolism, glutathione metabolism and bile metabolism returned to normal, and the adenosine 5’-monophosphate-activated protein kinase pathway was found to be activated in the HS group. Thus, this experiment uses metabolomics to reveal how and why steroidal saponins can alleviate largemouth bass heat stress, providing a new strategy for largemouth bass to face summer heat.

Source: Aquaculture Reports

DOI: 10.1016/j.aqrep.2023.101875