Get started
Work

Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus

Year: 2023
Type: article
Abstract: Adult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.
Source: Stem Cells
DOI: 10.1093/stmcls/sxad091
Authors: Ahmed Ismaeel, Jensen Goh, C Brooks Mobley, Kevin A Murach, Jamie O Brett, Antoine de Morrée, Thomas A Rando, Charlotte A Peterson, Yuan Wen, John J McCarthy
Institutions: University of Kentucky, Auburn University, University of Arkansas at Fayetteville, Stanford Medicine, Stanford University, University of California, Los Angeles
Language: English
Open Access status: hybrid
Estimated APC paid: $4,140
Awards: G2384062904, Myonuclear Epigenetics of Skeletal Muscle Mass Regulation with Age, G4209824390, G5336289650, G5770958815, G6026914104, G6890898177, G9000053072, G9000341954, G9000347284, Aging and Stem Cell Resilience
OpenAlex ID: W4389514287
FWCI: 1.564
Cites: 97
Cited by: 21
Related to: 10