Year: 2023
Type: preprint
Abstract: This process instruction describes steps for whole genome amplicon sequencing of SARS-CoV-2 from wastewater, including two approaches for concentration and RNA extraction steps (using liquid wastewater, or wastewater solids), purification steps, reverse transcription and PCR with ARTIC primers, library construction, and Illumina sequencing. Sequencing data produced using this protocol may be used for a variety of analyses including estimation of variant and mutation abundance. The read lengths are sufficient for read merging and analysis of intact amplicons. This protocol has been used for sequencing activities as part of the WastewaterSCAN program.
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4389660304