Work

Incorporating direct molecular diagnostics in management algorithms for nontuberculous mycobacteria: Is it high time?

Year: 2023

Type: review

Abstract: complex (MTBC) that cause pulmonary disease that is similar to the disease caused by MTBC. International guidelines for the diagnosis of pulmonary NTM disease are rigid and have remained unchanged for nearly 2 decades. In this opinion piece, we provide a new perspective on the traditional criteria by suggesting a diagnostic algorithm that incorporates direct molecular identification of NTM performed on raw sputum specimens (using Sanger or targeted deep sequencing approaches, among others) paired with traditional culture methods. Our approach ensures a more rapid diagnosis of pulmonary NTM disease, thus, facilitating timeous clinical diagnosis, and prompt treatment initiation, where indicated, and leverages recent advances in novel molecular techniques into routine NTM identification practice.

Source: IJID Regions

DOI: 10.1016/j.ijregi.2023.12.003

Authors: Christoffel Johannes Opperman, Sarishna Singh, Wynand Goosen, Helen Cox, Rob Warren, Aliasgar Esmail