Detection of HBV-DNA by Two PCR Methods in HBsAg Positive Sera, and Comparison of the Results with Serological Markers
Year: 1996
Type: article
Abstract: In this study, HBV-DNA was analysed by two different Polymerase Chain Reaction (PCR) methods in randomly selected 80 HBsAg positive sera, which were previously detected as serologicaly HBV positive in Department of Microbiology of ‹nönü University School of Medicine. HBV-DNA positivity was found by proteinase-K/phenol method (method I) in 25 of 80 sera (31.3%). HBV-DNA was detected in 23 sera (28.8%) by method II in which commercial kit was used (microDNA isolation kit, Strategene and Digene Sharp Signal System kit ). In all of 18 HBeAg positive sera (100%), HBV-DNA was detected by both of the methods. HBV-DNA positivity was detected in 7 of 62 anti-HBe positive sera (11.3%) and in 5 out of same samples (8.1%) by method I and II, respectively. There was no significant difference between the results of the two methods (p> 0.05). As a results, PCR laboratories can choose the methods appropriate for there facilities in detecting HBVDNA.
Institution: Inonu University
Language: English
Topic: Hepatitis B Virus Studies
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4389930928