Work

Polymerase-tethered nucleotides for nucleic acid synthesis

Year: 2024

Type: article

Abstract: Provided herein, among other things, is a conjugate comprising a polymerase and a nucleoside triphosphate, where the polymerase and the nucleoside triphosphate are covalently linked via a linker that comprises a cleavable linkage. A set of such conjugates, where the conjugates correspond to G, A, T (or U) and C is also provided. Methods for synthesizing a nucleic acid of a defined sequence are also provided. The conjugates can also be used for sequencing applications.

Source: OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Author: Arlow, Daniel; Palluk, Sebastian

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4390164033

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