Year: 2023
Type: review
Abstract: Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In addition to other components, CSF contains extracellular vesicles (EVs) that carry bioactive cargoes (e.g., lipids, nucleic acids, proteins), and that can have biological functions within and beyond the CNS. Thus, CSF EVs likely serve as both mediators of and contributors to communication in the CNS. Accordingly, their potential as biomarkers for CNS diseases has stimulated much excitement for and attention to CSF EV research. However, studies on CSF EVs present unique challenges relative to EV studies in other biofluids, including the invasive nature of CSF collection, limited CSF volumes and the low numbers of EVs in CSF as compared to plasma. Here, the objectives of the International Society for Extracellular Vesicles CSF Task Force are to promote the reproducibility of CSF EV studies by providing current reporting and best practices, and recommendations and reporting guidelines, for CSF EV studies. To accomplish this, we created and distributed a world-wide survey to ISEV members to assess methods considered 'best practices' for CSF EVs, then performed a detailed literature review for CSF EV publications that was used to curate methods and resources. Based on responses to the survey and curated information from publications, the CSF Task Force herein provides recommendations and reporting guidelines to promote the reproducibility of CSF EV studies in seven domains: (i) CSF Collection, Processing, and Storage; (ii) CSF EV Separation/Concentration; (iii) CSF EV Size and Number Measurements; (iv) CSF EV Protein Studies; (v) CSF EV RNA Studies; (vi) CSF EV Omics Studies and (vii) CSF EV Functional Studies.
DOI: 10.1002/jev2.12397
Authors: Ursula S. Sandau, Setty M. Magaña, Júlia Costa, John P. Nolan, Tsuneya Ikezu, Laura J. Vella, Hannah K. Jackson, Lissette Retana Moreira, Paola Loreto Palacio, Andrew F. Hill, Joseph F. Quinn, Kendall R. Van Keuren‐Jensen, Trevor J. McFarland, Joanna Palade, Eric A. Sribnick, Huaqi Su, Kostas Vekrellis, Beth Coyle, You Yang, Juan M. Falcón‐Perez, Rienk Nieuwland, Julie A. Saugstad, International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force
Institutions: Oregon Health & Science University, Nationwide Children's Hospital, Abigail Wexner Research Institute, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Scintillon Institute, Mayo Clinic in Florida, The Royal Melbourne Hospital, The University of Melbourne, Florey Institute of Neuroscience and Mental Health, University of Cambridge, Universidad de Costa Rica, La Trobe University, Victoria University, Portland VA Medical Center, Translational Genomics Research Institute, The Ohio State University, Academy of Athens, Biomedical Research Foundation of the Academy of Athens, University of Nottingham, Ikerbasque, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Amsterdam UMC Location University of Amsterdam, Amsterdam University Medical Centers, University of Amsterdam
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,200
Awards: G3340144160, G4941345466, Ciências da Vida para um Futuro Saudável e Sustentável, iNOVA4Health - Programa de Medicina Translacional (iBET, CEDOC/FCM, IPOLFG e ITQB), G6412162175, Targeting emerging P2RX7 signaling pathways in animal models of Alzheimer's disease, APOE and microglia-mediated progression in tau pathology in AD, Establishing MicroRNA Biomarkers for Diagnosing Alzheimer's Disease & Predicting Progression, G7858748947, iNOVA4Health - Programa de Medicina Translacional (iBET, CEDOC/FCM, IPOLFG e ITQB), Molecular characterization of extracellular vesicles for the spread of misfolded tau protein, Oregon Alzheimer's Disease Research Center, Human Cerebrospinal Fluid Extracellular Vesicles: Utility as Disease Specific Biomarkers and Impact on Alzheimer's Disease Pathology
OpenAlex ID: W4390449944