Characterization of Immunologically Distinct Pyruvate Dehydrogenase E1α Subunit Present in Rat Testis
Year: 1994
Type: article
Abstract: The tissue distribution of the pyruvate dehydrogenase complex (PDC) in several rat tissues was examined. Different amounts of immunoreactive proteins of pyruvate dehydrogenase (PDH) E1α E1β, E2, and E3 subunits were observed in five rat tissues with a decreasing order of PDC activities in heart > kidney > brain > liver > testis. Most subunits of the PDC appeared to be normal in size and immunoreactivities toward their corresponding polyclonal antibodies. However, virtually no immunoreactive PDH E1α subunit (Mr 41,000) was detected in testis while it was readily detected in other tissues examined, suggesting that the PDH E1α subunit present in rat testis may represent an immunologically distinct isoform. The testis-specific PDH E1α isoform was confirmed by immunoblot analysis using polyclonal antibodies against a synthetic peptide with the amino acid sequence around two major phosphorylation sites of PDH E1α subunit. Unlike the regular PDH E1α subunit (Mr 41,000) in the heart and kidney, the apparent molecular mass of PDH E1α isoform in testis of all four rat strains examined appeared to be 56,000 Da on SDS-polyacrylamide gel electrophoresis, representing a new class of PDH E1α subunit.
Source: Molecules and Cells
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,500
OpenAlex ID: W4390457353