Nucleotide Sequence of a Gene for Chlorophyll a/b Binding Protein of Rice
Year: 1993
Type: article
Abstract: To study the regulatory mechanism of gene expression and the transport mechanism of chlorophyll a/b binding protein of photosystem II (CABII) into chloroplast, a cDNA clone and its genomic clone were isolated. cDNA was synthesized from mRNA isolated from light-grown rice leaves, specifically amplified by polymerase chain reaction and was cloned directly into pUC!8 vector. The nucleotide sequence of the cDNA clone, rcabII, was determined. It contains 795 bp of an open reading frame encoding a polypeptide of 265 amino acids, with 7 bp of 5’-noncoding and 186 bp of 3’-noncoding sequences. The genomic clone for rcabII gene, grcabII, was isolated. It contains 795-bp of coding sequence, which is identical to that of the cDNA clone rcabII, and its 5 - and 3’-flanking regions. The coding region of grcabII is not interrupted by intervening sequence. Genomic Southern blot analysis suggested that the rcabII gene is a member of relatively small multigene family in the rice genome. A comparison of the deduced amino acid sequences with that of other cabll genes shows that the rcabII gene belongs to type I of cabll gene. Mature protein encoded by rcabII contains three hydrophobic transmembrane domains and the transit peptide which has a theoretical propensity to form amphiphilic α-helical structure. Northern blot analysis revealed that mRNA size for the cloned cDNA is 1.1 kb long and its gene expression is regulated at transcriptional level in light-inducible manner. In the 5’ upstream regions of grcabII, conserved sequences in light responsive genes are found such as the box III*, box II, G-box, 3AF1-binding site, an octamer repeat and phytochrome-responsive element. The function of these putative regulatory elements are discussed.
Source: Molecules and Cells
Authors: Min Gyu Lee, Yun Jung Kang, Chung Ho Kim, Jin Nam Choi, Han-Jip Kim, Jong Seob Lee, Kwang-Woong Lee, Yang Do Choi
Institutions: Seoul National University, Ajou University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,500
OpenAlex ID: W4390461806