Cbl Competitively Inhibits Epidermal Growth Factor-induced Activation of Phospholipase C-γ1
Year: 2003
Type: article
Abstract: Phospholipase C-γ1 (PLC-γ1) plays pivotal roles in cellular growth and proliferation through its two Src homology (SH) 2 domains and its single SH3 domain, which interact with signaling molecules in response to various growth factors and hormones. However, the role of the SH domains in the growth factor-induced regulation of PLC-γ1 is unclear. By peptide-mass fingerprinting analysis we have identified Cbl as a binding protein for the SH3 domain of PLC-γ1 from rat pheochromatocyte PC12 cells. Association of Cbl with PLC-γ1 was induced by epidermal growth factor (EGF) but not by nerve growth factor (NGF). Upon EGF stimulation, both Cbl and PLC-γ1 were recruited to the activated EGF receptor through their SH2 domains. Mutation of the SH2 domains of either Cbl or PLC-γ1 abrogated the EGF-induced interaction of PLC-γ1 with Cbl, indicating that SH2-mediated translocation is essential for the association of PLC-γ1 and Cbl. Overexpression of Cbl attenuated EGF-induced tyrosine phosphorylation and the subsequent activation of PLC-γ1 by interfering competitively with the interaction between PLC-γ1 and EGFR. Taken together, these results provide the first indications that Cbl may be a negative regulator of intracellular signaling following EGF-induced PLC-γ1 activation.
Source: Molecules and Cells
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,500
OpenAlex ID: W4390473176