Work

Comparison and agreement between two image analysis tools for quantifying GFP::SNB-1 puncta in fshr-1 mutants of C. elegans

Year: 2023

Type: article

Abstract: and wild type worms. Based on these findings, we conclude that the Fiji platform is viable as a method for analyzing synaptic vesicle localization and abundance at cholinergic dorsal nerve cord motor NMJs and expect the Fiji puncta plugin to be of broad utility in imaging across a variety of imaging platforms and synaptic markers.

Source: PubMed Central

DOI: 10.17912/micropub.biology.001005

Authors: Heino Hulsey-Vincent, Makenzi McClain, Morgan Buckley, Jennifer R. Kowalski, Caroline L. Dahlberg

Institutions: Western Washington University, Butler University

Language: English

Topic: Cell Image Analysis Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Western Washington University, National Institutes of Health, National Institute of Neurological Disorders and Stroke

Awards: Caenorhabditis Genetics Center, Investigation of the role of the G protein-coupled receptor FSHR-1 in multi-tissue control of neuromuscular signaling in normal and oxidative stress conditions