FIGURE 7 from WNT4 Regulates Cellular Metabolism via Intracellular Activity at the Mitochondria in Breast and Gynecologic Cancers
Year: 2024
Type: other
Abstract: WNT4 variant genotype is associated with active WNT4 signaling and metabolic remodeling in ovarian cancer cells. A, Left, Proliferation assessed by dsDNA quantification 6 days post-transfection with siRNA (siNT = non-targeting control pool). Bars represent mean of 6 biological replicates ± SD; *, P < 0.05, siWNT4 versus siNT, t test with Welch correction. WT versus Var model comparison by t test of mean fold changes for siWNT4 versus siNT. Right, WNT4 mRNA by qPCR in parallel samples, 72 hours after siRNA. Mean of biological triplicate, fold change versus siNT. B, Lysates harvested 72 hours posttransfection. MCL1 levels assessed by densitometry and normalized to total S6 loading control (6). Fold change in normalized MCL1 levels in Variant versus WT genotype cell lines compared by Student t test. C, Metabolites dysregulated 72 hours after WNT4 knockdown (n = 44), as for MM134. D, Fold changes in lipid metabolites. Dashed line on y-axis: FDR = 0.2. E, Pathway enrichment (Metaboanalyst) for metabolites shown in C; K = KEGG, S = SMPDB.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4390944598