Morphoanatomical, pharmacotaxonomical, physiochemical and phytochemical profiles, including TLC and HPTLC analysis of Ipomoea pes-tigridis L.
Year: 2023
Type: article
Abstract: Herbal drugs are becoming more popular around the world, particularly in developed countries, but one of the barriers to their acceptance is the lack of a standard quality control profile.The main goal of this study is to look at the morphoanatomical, pharmacotaxonomical, physiochemical, and phytochemical properties of the leaves of Ipomoea pes-tigridis L. The study represents macroscopical and microscopical evaluation along with estimation of its physicochemical parameters, such as ash values, extractive values, fluorescence analysis, and phytochemical screening, using WHO-prescribed standard procedures.The phytochemical screening includes preliminary phytochemical screening, thin-layer chromatography, and high-performance thin-layer chromatographic analysis.All the pharmacognostic standardisation parameters analysed gave satisfactory results.The preliminary phytochemical screening of various extracts of I. pestigridis showed the presence of various phytoconstituents such as carbohydrates, proteins, terpenoids, flavonoids, phenolic compounds, and resins.The thin layer chromatography (TLC) analysis of the hydroalcoholic extract showed the presence of flavonoids and polyphenolic compounds from the Rf values.Equally, the high performance thin layer chromatography (HPTLC) analysis showed that the 3D finger print profile of TLC reflects 8 peaks and 12 peaks at 254 nm and 366 nm, respectively.Among the spots produced in HPTLC, the Rf value of 0.7 may indicate flavonoid content.Hence, these findings reveal a standardisation profile for I. pes-tigridis, which would be useful in botanical identification and plant drug authentication, as well as in the preparation of herbal monographs and the prevention of adulteration.Eventually, the presence of phytocontituents may have a synergistic effect on the therapeutic potential and bioactivity of the plant.
Authors: S. Sameemabegum, T. Prabha, S. Sribhuvaneswari, T. Sivakumar
Institution: Nandha College of Pharmacy
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4390979096