FIGURE 1 from FASN Gene Methylation is Associated with Fatty Acid Synthase Expression and Clinical-genomic Features of Prostate Cancer
Year: 2024
Type: other
Abstract: FASN protein is upregulated and FASN gene is hypomethylated in tumor compared with benign tissue in JHU primary tumor cohort. A, Dual FASN and p63 IHC assay in representative primary prostate tumor from JHU cohort (scale bar = 250 µm). FASN and p63 are both labeled in brown. Benign glands with nuclear p63 labeling in basal cells (arrow) are then manually excluded from analysis and FASN scoring is performed on tumor glands only. See Supplementary Fig. S2. B, Quantified FASN protein expression by immunostaining in benign prostate glands versus tumor glands from JHU cohort; each point represents the mean H-score from a single prostatectomy sample, analyzed in a matched analysis. C, Representative plots of beta values (range: 0–1) by FASN probe on InfiniumEPIC for a representative paired benign (blue) and tumor (red) sample set from the JHU cohort. TSS: transcription start site; UTR: untranslated region. D,FASN gene methylation beta values by FASN probe for benign tissue versus tumor tissue in JHU cohort. E, Mean FASN gene methylation probe beta values for benign tissue versus tumor tissue in JHU cohort. Each point represents the mean beta value from a single prostatectomy sample analyzed in a matched analysis (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4391011795