Work

Deciphering the crystal structure of a novel nanobody against the NEIL1 DNA glycosylase

Year: 2024

Type: article

Abstract: Nanobodies (VHHs) are single-domain antibodies with three antigenic CDR regions and are used in diverse scientific applications. Here, an ∼14 kDa nanobody (A5) specific for the endonuclease VIII (Nei)-like 1 or NEIL1 DNA glycosylase involved in the first step of the base-excision repair pathway was crystallized and its structure was determined to 2.1 Å resolution. The crystals posed challenges due to potential twinning and anisotropic diffraction. Despite inconclusive twinning indicators, reprocessing in an orthorhombic setting and molecular replacement in space group P 2 1 2 1 2 enabled the successful modeling of 96% of residues in the asymmetric unit, with final R work and R free values of 0.199 and 0.229, respectively.

Source: Acta Crystallographica Section D Structural Biology

DOI: 10.1107/s205979832400038x

Authors: Marlo K. Thompson, Nidhi Sharma, Andrea Thorn, Aishwarya Prakash

Institutions: USA Mitchell Cancer Institute, University of South Alabama, Universität Hamburg

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: green