FIGURE 6 from Genome-wide p63-Target Gene Analyses Reveal TAp63/NRF2-Dependent Oxidative Stress Responses
Year: 2024
Type: other
Abstract: TAp63 and NRF2 coordinately regulate the expression of TAp63-specific target genes. A, qRT-PCR for the indicated TAp63-specific target genes in epidermal cells of the indicated genotype. Data are mean ± SD, n = 3, * versus WT, P < 0.005, two-tailed t test. B, qRT-PCR of TAp63 ChIP assays using epidermal cells of the indicated genotype on the TAp63-specific peaks of the indicated TAp63-specific target genes. Data are mean ± SD, n = 3, * versus WT, P < 0.005, two-tailed t test. C, qRT-PCR of Nrf2 ChIP-re-ChIP assays using the TAp63 chromatin immunoprecipitated genomic regions of the indicated TAp63-specific target genes from epidermal cells of the indicated genotype. Data are mean ± SD, n = 3. * versus WT, P < 0.005, two-tailed t test. D, qRT-PCR of the indicated TAp63-specific target genes in WT, ΔNp63−/−, and TAp63−/− epidermal cells transfected with the indicated siRNAs. Data are mean ± SD, n = 3, * vs. WT siControl, # versus WT siNrf2, P < 0.005, two-tailed t test. E, Model depicting the coordinated regulation of TAp63-specific metabolic genes by TAp63 and NRF2.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4391449745