FIGURE 5 from Genome-wide p63-Target Gene Analyses Reveal TAp63/NRF2-Dependent Oxidative Stress Responses
Year: 2024
Type: other
Abstract: ∆Np63 and TAp63 control the expression of their common target genes together with Stat proteins. A, qRT-PCR for the indicated ΔNp63 and TAp63 common target genes in epidermal cells of the indicated genotype. Data are mean ± SD, n = 3, * versus WT, P < 0.005, two-tailed t test. B, qRT-PCR of ∆Np63 ChIP assays using epidermal cells of the indicated genotype on the common peaks of the indicated common target genes. Data are mean ± SD, n = 3, * versus WT, P < 0.005, two-tailed t test. C, qRT-PCR of TAp63 ChIP assays using epidermal cells of the indicated genotype on the same genomic regions as in B. Data are mean ± SD, n = 3, * versus WT, P < 0.005, two-tailed t test. D, qRT-PCR of Stat4 ChIP-re-ChIP assays using the ∆Np63 chromatin immunoprecipitated genomic regions of the indicated common target genes from epidermal cells of the indicated genotype. Data are mean ± SD, n = 3. * versus WT, P < 0.005, two-tailed t test. E, qRT-PCR of Stat4 ChIP-re-ChIP assays using the TAp63 chromatin immunoprecipitated genomic regions of the indicated common target genes from epidermal cells of the indicated genotype. Data are mean ± SD, n = 3. * versus WT, P < 0.005, two-tailed t test. F, qRT-PCR of the indicated common target genes in WT, TAp63−/−, and ΔNp63−/− epidermal cells transfected with the indicated siRNAs. Data are mean ± SD, n = 3, * versus WT siControl, # versus WT siStat4, and $ versus WT siStat5a, P < 0.005, two-tailed t test. G, Model depicting the coordinated regulation of ΔNp63 and TAp63 common target genes with the Stat family members, Stat4 and Stat5a.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4391449815