FIGURE 4 from Genome-wide p63-Target Gene Analyses Reveal TAp63/NRF2-Dependent Oxidative Stress Responses
Year: 2024
Type: other
Abstract: ∆Np63 cooperates with the FOX family members to regulate cell motility genes. A, qRT-PCR for the indicated ΔNp63-specific target genes in epidermal cells of the indicated genotype. Data are mean ± SD, n = 3, * versus WT, P < 0.005, two-tailed t test. B, qRT-PCR of ∆Np63 ChIP assays using epidermal cells of the indicated genotype on the ∆Np63-specific peaks of the indicated ΔNp63-specific target genes. Data are mean ± SD, n = 3, * versus WT, P < 0.005, two-tailed t test. C, qRT-PCR of Foxj2 ChIP-re-ChIP assays using the ∆Np63 chromatin immunoprecipitated genomic regions of the indicated ΔNp63-specific target genes from epidermal cells of the indicated genotype. Data are mean ± SD, n = 3. * versus WT, P < 0.005, two-tailed t test. D, qRT-PCR of the indicated ΔNp63-specific target genes in WT, ΔNp63−/−, and TAp63−/− epidermal cells transfected with the indicated siRNAs. Data are mean ± SD, n = 3, * versus WT siControl, # versus WT siFoxj2, and $ versus WT siFoxl1, P < 0.005, two-tailed t test. E, Model depicting the coordinated regulation of ∆Np63-specific cell motility genes by ∆Np63 and the FOX family members, FoxJ2 and Foxl1. F, Representative Western blot analysis using the indicated antibodies of the endogenous interacting proteins immunoprecipitated with p63 isoform–specific antibodies. IgGs from normal rabbit serum were used as negative control. Protein inputs (5% of lysates) are also shown.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4391449889