0673 - Human Mesenchymal Stem Cell Morphology, Migration, and Differentiation on Micro and Nano-Textured Titanium
Year: 2019
Type: other
Abstract: Cell-material interactions are of particular interest in biomedical implants because early events between the cells and the biomaterial can define the success of the device. Upon implantation, bacteria compete with mesenchymal stem cells to attach at the implant surface resulting in either biofilm formation or bone tissue integration. The chosen material and surface topography are an integral part of this initial interaction. Previous work has demonstrated the topography of Ti6Al4V has the ability to regulate the early attachment, migration and differentiation of human mesenchymal stem cells (hMSCs). Specifically, titanium surfaces exhibiting macro- micro roughness resulted in improved cell spreading, random migration, and increased differentiation towards an osteoblastic lineage when compared with smooth topographies (both Ti6Al4V and PEEK). The goal of the current investigation was to further characterize the morphology, migration and differentiation of hMSCs on smooth topographies (both Ti6Al4V and PEEK), macro-micro rough topographies (Endoskeleton) and macro-micro-nano rough topographies (nanoLOCK). The primary hypothesis is that the introduction of nano-topography on micro-rough Ti6Al4V will promote improved cell attachment, migration and differentiation. A secondary hypothesis seeks to identify a correlation between the hMSC morphology at early time-points and long-term differentiation.Together the data presented will validate our two initial hypotheses. Notably, adding a nano-roughness to micro-roughened Ti6Al4V improves the velocity of migrating hMSCs in addition to the increasing the rate at which the hMSCs differentiate to osteoblasts. Furthermore, this improved differentiation appears to be correlated to a rapid adoption of a stellate morphology by the hMSCs on the nanoLOCK surfaces. Thus, these results support the combined macro-micro-nano topography of the nanoLOCK surface as being a substantial improvement over all other spinal fusion cage materials and topographies as demonstrated through rapid random migration necessary to cover a surface, the evolution of a morphology typical of mature osteoblasts/osteocytes, and a rapid progression through the early and mid-osteogenic differentiation markers, ALP and OSX respectively.
Authors: Justin Brown, Jennifer M., Michelle B.
Institution: Pennsylvania State University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4391532529