1492 - In Vitro Response to Implants with Internally Roughened Micron and Nano Features
Year: 2019
Type: other
Abstract: Spinal fusion surgery combines two or more vertebrae together to reduce discomfort by immobilizing a painful vertebral motion segment and restoring spinal stability. The insertion of the interbody fusion device serves to support the space previously occupied by an intervertebral disc and, based on the material and topography, can also facilitate new bone formation. Recent research has focused on enhancing the endplate-contacting surfaces of interbody fusion devices and has demonstrated that micro-nano topography combined with the macro-roughness of the anti-expulsion surface can enhance stem cell morphology and differentiation. However, an often overlooked feature of fusion devices is the topography of the internal walls of interbody spinal implants which may play an important role during bone graph integration and fusion. This evaluation examines whether uniform application of surface topography at a cellular level invokes a similar cyto/chemokines expression and phenotype when exposed to mesenchymal stem cells (MSCs), regardless of location on the device.The results presented in this study demonstrate equivalent hMSC gene expression and morphology on the interior and exterior surfaces of devices with uniform topography at a cellular level. Specifically, the nanoLOCKu00ae surface induced an osteoblastic phenotype in hMSCs on both surfaces, whereas the smooth titanium and PEEK demonstrated a fibroblastic morphology across both interior and exterior surfaces. The cell morphology on the nanoLOCKu00ae surface correlated with the production of osteogenic cyto and chemokines, while PEEK promoted upregulation of inflammatory cyto and chemokines.
Authors: Justin Brown, Jennifer M., Michelle B.
Institution: Pennsylvania State University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4391553190