Author response: Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression
Year: 2022
Type: peer-review
Abstract: Out of the several hundred copies of rRNA genes arranged in the nucleolar organizing regions (NOR) of the five human acrocentric chromosomes, ~50% remain transcriptionally inactive.NOR-associated sequences and epigenetic modifications contribute to the differential expression of rRNAs.However, the mechanism(s) controlling the dosage of active versus inactive rRNA genes within each NOR in mammals is yet to be determined.We have discovered a family of ncRNAs, SNULs (Single NUcleolus Localized RNA), which form constrained sub-nucleolar territories on individual NORs and influence rRNA expression.Individual members of the SNULs monoallelically associate with specific NOR-containing chromosomes.SNULs share sequence similarity to pre-rRNA and localize in the sub-nucleolar compartment with pre-rRNA.Finally, SNULs control rRNA expression by influencing pre-rRNA sorting to the DFC compartment and pre-rRNA processing.Our study discovered a novel class of ncRNAs influencing rRNA expression by forming constrained nucleolar territories on individual NORs.
Authors: Qinyu Hao, Minxue Liu, Swapna Vidhur Daulatabad, Saba Gaffari, You Jin Song, Rajneesh Srivastava, Shivang Bhaskar, Anurupa Moitra, Hazel Mangan, Elizabeth Tseng, Rachel B Gilmore, Susan M Frier, Xin Chen, Chengliang Wang, Sui Huang, Stormy Chamberlain, Hong Jin, Jonas Korlach, Brian McStay, Saurabh Sinha, Sarath Chandra Janga, Supriya G Prasanth, Kannanganattu V Prasanth
Institutions: University of Illinois Urbana-Champaign, Indiana University – Purdue University Indianapolis, Ollscoil na Gaillimhe – University of Galway, Pacific Biosciences (United States), University of Connecticut, Ionis Pharmaceuticals (United States), Northwestern University, Northwestern University, Carl R. Woese Institute for Genomic Biology, Georgia Institute of Technology, Cancer Center at Illinois
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Awards: G1427604142, G2662092234, Characterization of nuclear-retained RNA-mediated gene regulatory mechanisms, SHF: Small: Automated Algorithm/Hardware Co-Design for Accelerating Nanopore Base-calling, G4570321760, G5037515237, G5179224734, Developing TSA-RNA-seq for subcellular transcriptomics, G6018716503, Role of BEN domain proteins in chromatin organization and gene expression, G6777000098, CAREER: Establishment of Replication Timing and Patterning in Mammalian Cells, EAGER: Algorithmic frameworks and resources for mapping RNA modifications from single molecule direct RNA-sequencing data, G7688728642, Quantitative regulatory genomics: networks, cis-regulatory codes, and phenotypic variation, Science and Technology Center for Quantitative Cell Biology, G9000237378, G9000264727, G9000340136, G9000341019, G9000342712
OpenAlex ID: W4391643626