Editor's evaluation: The tRNA thiolation-mediated translational control is essential for plant immunity
Year: 2023
Type: peer-review
Abstract: Plants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections.However, how the translation system contributes to plant immunity is largely unknown.The evolutionarily conserved thiolation modification of transfer RNA (tRNA) ensures efficient decoding during translation.Here, we show that tRNA thiolation is required for plant immunity in Arabidopsis.We identify a cgb mutant that is hyper-susceptible to the pathogen Pseudomonas syringae.CGB encodes ROL5, a homolog of yeast NCS6 required for tRNA thiolation.ROL5 physically interacts with CTU2, a homolog of yeast NCS2.Mutations in either ROL5 or CTU2 result in loss of tRNA thiolation.Further analyses reveal that both transcriptome and proteome reprogramming during immune responses are compromised in cgb.Notably, the translation of salicylic acid receptor NPR1 is reduced in cgb, resulting in compromised salicylic acid signaling.Our study not only reveals a regulatory mechanism for plant immunity but also uncovers an additional biological function of tRNA thiolation. Editor's evaluationThis valuable study provides solid evidence for a role of tRNA thiolation in Arabidopsis immunity through genetic, transcriptomic, and proteomic approaches, specifically that the tRNA mcm5s2U modification affects SA signaling through NPR1 translation.
Source: eLife
Author: Detlef Weigel
Institution: Max Planck Institute for Biology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,500
OpenAlex ID: W4391792972